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timelapse analysis software  (Biosite Inc)

 
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    Biosite Inc timelapse analysis software
    Bacterial cultures of EPEC or E. coli K-12 were grown overnight in LB media (Non-Activation) or in DMEM for 3 hr at 37°C to OD 600 ~0.3 (Activation). Cultures were then diluted, plated on LB agar, and incubated at 32°C (non-activating conditions). Colony appearance time was monitored by ScanLag at 15 min intervals. The resulting histograms show ( A , B , C , D ) the fractions of colonies detected at each time point and ( E , F ) colony size distributions 1000 min after plating. Colonies larger or smaller than 105 pixels were defined as ‘BIG’ and ‘SMALL’ morphotypes, respectively. ( A – C , E , F ) Experiments were repeated in at least four independent biological replicates. ( D ) shows the cumulative of 4 independent biological replicates. ( G ) Time-lapse microscopy phase-contrast images of the two co-existing morphotypes, BIG and SMALL. Time points are indicated. Similar results were obtained in at least 10 different locations and in two independent biological replicates. Scale bar: 50 μm. DOI: http://dx.doi.org/10.7554/eLife.19599.003 10.7554/eLife.19599.004 Figure 1—source data 1. This Source Data file contains appearance time histogram raw data for (activated and non-activated EPEC cultures) from ScanLag experiments. Data collected from four experiments. The data were collected by the ScanningManager software application and analyzed by <t>TimeLapse</t> analysis software application http://bio-site.phys.huji.ac.il/Materials. For figures histogram was fitted to total 100% bacteria. Data columns marked with * and ** are used for creating respectively. DOI: http://dx.doi.org/10.7554/eLife.19599.004
    Timelapse Analysis Software, supplied by Biosite Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/timelapse analysis software/product/Biosite Inc
    Average 90 stars, based on 1 article reviews
    timelapse analysis software - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "A long-term epigenetic memory switch controls bacterial virulence bimodality"

    Article Title: A long-term epigenetic memory switch controls bacterial virulence bimodality

    Journal: eLife

    doi: 10.7554/eLife.19599

    Bacterial cultures of EPEC or E. coli K-12 were grown overnight in LB media (Non-Activation) or in DMEM for 3 hr at 37°C to OD 600 ~0.3 (Activation). Cultures were then diluted, plated on LB agar, and incubated at 32°C (non-activating conditions). Colony appearance time was monitored by ScanLag at 15 min intervals. The resulting histograms show ( A , B , C , D ) the fractions of colonies detected at each time point and ( E , F ) colony size distributions 1000 min after plating. Colonies larger or smaller than 105 pixels were defined as ‘BIG’ and ‘SMALL’ morphotypes, respectively. ( A – C , E , F ) Experiments were repeated in at least four independent biological replicates. ( D ) shows the cumulative of 4 independent biological replicates. ( G ) Time-lapse microscopy phase-contrast images of the two co-existing morphotypes, BIG and SMALL. Time points are indicated. Similar results were obtained in at least 10 different locations and in two independent biological replicates. Scale bar: 50 μm. DOI: http://dx.doi.org/10.7554/eLife.19599.003 10.7554/eLife.19599.004 Figure 1—source data 1. This Source Data file contains appearance time histogram raw data for (activated and non-activated EPEC cultures) from ScanLag experiments. Data collected from four experiments. The data were collected by the ScanningManager software application and analyzed by TimeLapse analysis software application http://bio-site.phys.huji.ac.il/Materials. For figures histogram was fitted to total 100% bacteria. Data columns marked with * and ** are used for creating respectively. DOI: http://dx.doi.org/10.7554/eLife.19599.004
    Figure Legend Snippet: Bacterial cultures of EPEC or E. coli K-12 were grown overnight in LB media (Non-Activation) or in DMEM for 3 hr at 37°C to OD 600 ~0.3 (Activation). Cultures were then diluted, plated on LB agar, and incubated at 32°C (non-activating conditions). Colony appearance time was monitored by ScanLag at 15 min intervals. The resulting histograms show ( A , B , C , D ) the fractions of colonies detected at each time point and ( E , F ) colony size distributions 1000 min after plating. Colonies larger or smaller than 105 pixels were defined as ‘BIG’ and ‘SMALL’ morphotypes, respectively. ( A – C , E , F ) Experiments were repeated in at least four independent biological replicates. ( D ) shows the cumulative of 4 independent biological replicates. ( G ) Time-lapse microscopy phase-contrast images of the two co-existing morphotypes, BIG and SMALL. Time points are indicated. Similar results were obtained in at least 10 different locations and in two independent biological replicates. Scale bar: 50 μm. DOI: http://dx.doi.org/10.7554/eLife.19599.003 10.7554/eLife.19599.004 Figure 1—source data 1. This Source Data file contains appearance time histogram raw data for (activated and non-activated EPEC cultures) from ScanLag experiments. Data collected from four experiments. The data were collected by the ScanningManager software application and analyzed by TimeLapse analysis software application http://bio-site.phys.huji.ac.il/Materials. For figures histogram was fitted to total 100% bacteria. Data columns marked with * and ** are used for creating respectively. DOI: http://dx.doi.org/10.7554/eLife.19599.004

    Techniques Used: Activation Assay, Incubation, Time-lapse Microscopy, Software, Bacteria



    Similar Products

    timelapse analysis software  (Biosite Inc)
    90
    Biosite Inc timelapse analysis software
    Bacterial cultures of EPEC or E. coli K-12 were grown overnight in LB media (Non-Activation) or in DMEM for 3 hr at 37°C to OD 600 ~0.3 (Activation). Cultures were then diluted, plated on LB agar, and incubated at 32°C (non-activating conditions). Colony appearance time was monitored by ScanLag at 15 min intervals. The resulting histograms show ( A , B , C , D ) the fractions of colonies detected at each time point and ( E , F ) colony size distributions 1000 min after plating. Colonies larger or smaller than 105 pixels were defined as ‘BIG’ and ‘SMALL’ morphotypes, respectively. ( A – C , E , F ) Experiments were repeated in at least four independent biological replicates. ( D ) shows the cumulative of 4 independent biological replicates. ( G ) Time-lapse microscopy phase-contrast images of the two co-existing morphotypes, BIG and SMALL. Time points are indicated. Similar results were obtained in at least 10 different locations and in two independent biological replicates. Scale bar: 50 μm. DOI: http://dx.doi.org/10.7554/eLife.19599.003 10.7554/eLife.19599.004 Figure 1—source data 1. This Source Data file contains appearance time histogram raw data for (activated and non-activated EPEC cultures) from ScanLag experiments. Data collected from four experiments. The data were collected by the ScanningManager software application and analyzed by <t>TimeLapse</t> analysis software application http://bio-site.phys.huji.ac.il/Materials. For figures histogram was fitted to total 100% bacteria. Data columns marked with * and ** are used for creating respectively. DOI: http://dx.doi.org/10.7554/eLife.19599.004
    Timelapse Analysis Software, supplied by Biosite Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/timelapse analysis software/product/Biosite Inc
    Average 90 stars, based on 1 article reviews
    timelapse analysis software - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

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    Bacterial cultures of EPEC or E. coli K-12 were grown overnight in LB media (Non-Activation) or in DMEM for 3 hr at 37°C to OD 600 ~0.3 (Activation). Cultures were then diluted, plated on LB agar, and incubated at 32°C (non-activating conditions). Colony appearance time was monitored by ScanLag at 15 min intervals. The resulting histograms show ( A , B , C , D ) the fractions of colonies detected at each time point and ( E , F ) colony size distributions 1000 min after plating. Colonies larger or smaller than 105 pixels were defined as ‘BIG’ and ‘SMALL’ morphotypes, respectively. ( A – C , E , F ) Experiments were repeated in at least four independent biological replicates. ( D ) shows the cumulative of 4 independent biological replicates. ( G ) Time-lapse microscopy phase-contrast images of the two co-existing morphotypes, BIG and SMALL. Time points are indicated. Similar results were obtained in at least 10 different locations and in two independent biological replicates. Scale bar: 50 μm. DOI: http://dx.doi.org/10.7554/eLife.19599.003 10.7554/eLife.19599.004 Figure 1—source data 1. This Source Data file contains appearance time histogram raw data for (activated and non-activated EPEC cultures) from ScanLag experiments. Data collected from four experiments. The data were collected by the ScanningManager software application and analyzed by TimeLapse analysis software application http://bio-site.phys.huji.ac.il/Materials. For figures histogram was fitted to total 100% bacteria. Data columns marked with * and ** are used for creating respectively. DOI: http://dx.doi.org/10.7554/eLife.19599.004

    Journal: eLife

    Article Title: A long-term epigenetic memory switch controls bacterial virulence bimodality

    doi: 10.7554/eLife.19599

    Figure Lengend Snippet: Bacterial cultures of EPEC or E. coli K-12 were grown overnight in LB media (Non-Activation) or in DMEM for 3 hr at 37°C to OD 600 ~0.3 (Activation). Cultures were then diluted, plated on LB agar, and incubated at 32°C (non-activating conditions). Colony appearance time was monitored by ScanLag at 15 min intervals. The resulting histograms show ( A , B , C , D ) the fractions of colonies detected at each time point and ( E , F ) colony size distributions 1000 min after plating. Colonies larger or smaller than 105 pixels were defined as ‘BIG’ and ‘SMALL’ morphotypes, respectively. ( A – C , E , F ) Experiments were repeated in at least four independent biological replicates. ( D ) shows the cumulative of 4 independent biological replicates. ( G ) Time-lapse microscopy phase-contrast images of the two co-existing morphotypes, BIG and SMALL. Time points are indicated. Similar results were obtained in at least 10 different locations and in two independent biological replicates. Scale bar: 50 μm. DOI: http://dx.doi.org/10.7554/eLife.19599.003 10.7554/eLife.19599.004 Figure 1—source data 1. This Source Data file contains appearance time histogram raw data for (activated and non-activated EPEC cultures) from ScanLag experiments. Data collected from four experiments. The data were collected by the ScanningManager software application and analyzed by TimeLapse analysis software application http://bio-site.phys.huji.ac.il/Materials. For figures histogram was fitted to total 100% bacteria. Data columns marked with * and ** are used for creating respectively. DOI: http://dx.doi.org/10.7554/eLife.19599.004

    Article Snippet: The data were collected by the ScanningManager software application and analyzed by TimeLapse analysis software application http://bio-site.phys.huji.ac.il/Materials.

    Techniques: Activation Assay, Incubation, Time-lapse Microscopy, Software, Bacteria